In order to cross the blood brain barrier (BBB), liposomes were prepared for brain targeting due to their structural resemblance to the cell membrane. Since BBB has a negative charge, therefore forming neutral liposomes will favor their passive diffusion through such a barrier leading to drug delivery in the brain. Consequently, the addition of a positive charge inducer will shift the zeta potential of the formed negatively charged liposomes towards neutral or even positive zeta potential. However having a neutral charge makes liposomes more vulnerable to form aggregates leading to an increase in particle size, leaving the nano range. Tween 80, non ionic surfactant, therefore was added to give stability to the formed liposomes due to its steric hindrance effect. A 32 full factorial design was carried out to study the effect of the addition of increasing molar ratios of DDAB and Tween 80 on particle size, zeta potential and entrapment efficiency. Afterwards, numerical optimization was performed based on the utilization of desirability functions. Particle size analysis, zeta potential, entrapment efficiency and in-vitro drug release were studied for the optimized formula.
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